This research explores the use of ultrasound-assisted extraction as an eco-friendly approach for extracting collagen from the skin of Napoleon wrasse (Cheilinus undulatus), a species that has not been extensively studied for its collagen content. The main aim was to investigate a more sustainable and effective extraction method compared to conventional techniques. Proximate analysis of the fish sample indicated a protein content of 20.23 ± 0.40%, establishing a baseline for estimating the collagen content. Ultrasound extraction involved varying durations (0, 10, 20, 30 minutes) and different acid types (acetic, lactic, citric acid), with collagen yields evaluated for each condition. The results revealed that ultrasound-assisted extraction significantly increased collagen yield, with both extraction duration and acid type demonstrating a notable impact. The highest collagen yield (9.56 ± 1.41%) was obtained using citric acid, followed by lactic acid (5.99 ± 1.79%) and acetic acid (4.10 ± 0.35%). Collagen purity was confirmed through UV absorbance spectroscopy, which consistently showed a maximum absorption peak at 230 nm across all acid treatments. This study illustrates that ultrasound-assisted extraction can enhance collagen yield