In recent years, spirituality and religion have received growing attention within the field of psychology. Spirituality may yield a sense of ultimate purpose, providing stability and buffering against stress during the crisis time. Previous research has shown that neuroticism is the dimension of personality that is fundamental to individual differences. The present aim was to examine the relationship between a dimensional prayer measure and Eysenck's model of personality to ascertain if personality can be differentially predicted by prayer and to investigate difference in level of education and also gender. A random sample of 350 adults completed a form containing the abbreviated Eysenck Personality Questionnaire together with questions about the practice of prayer and level of education. While prayer was no associated with low neuroticism scores, low levels of literacy and gender were associated with high neuroticism scores. Two-Sample Kolmogorov-Smirnov Test and T- Test showed that ritual prayer was the only significant independent predictor of personality, predicting psychoticism.