The aim of this work is to design and synthetize a new anticancer drug having reduced toxicity and side-effect compared to the known anti-cancer drugs. The interaction of DNA with new anticancer Pd complex, namely [Pd(phendione) (n-butyl glycine)]NO3 (where phendione= 1,10-phenanthroline -5,6-dione) has been studied by UV spectroscopy in Tris-HCl buffer of pH 7.4 at 27 o C and 37 o C. These experiments revealed that the water soluble complex can denature DNA at very low concentrations (~1mM) and its ability increased by raising the temperature to 37 o C. Also, the thermodynamic parameters of these interactions are calculated and compared. Fluorescence studies show this complex can bind to DNA via intercalation.