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Crocin Protects Against Beta-Amyloid Peptide-Induced Cell Apoptosis in PC12 Cells Via the PI3 K Pathway
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- تاریخ انتشار 1401/12/12
- تعداد صفحات 14
- زبان مقاله انگلیسی
- حجم فایل 566 کیلو بایت
- تعداد مشاهده چکیده 111
- قیمت 29,000 تومان
- تخفیف 0 تومان
- قیمت با احتساب تخفیف: 29,000 تومان
- قیمت برای کاربران عضو سایت: 23,200 تومان
- محل انتشار چهاردهمین همایش ملی گیاهان دارویی وکشاورزی پایدار
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نویسندگان مقاله
- Reyhaneh Taheri Medical Toxicology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
- Elham Hadipour Targeted Drug Delivery Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran
- Zahra Tayarani-Najaran Targeted Drug Delivery Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran
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چکیده مقاله
Background: Crocin is a known compound with the antioxidant and anti-inflammatory properties which may help to reduce the progression of neurological disorders. In this study, we aimed to investigate the protective effects of crocin on beta-amyloid peptide Aβ (1-40) and hydrogen peroxide (H2O2) induced neurotoxicity in PC12 cells.
Methods: PC12 cells were pretreated with crocin and donepezil (5 and 10 μM) for 2 h and then treated with Aβ (1-40) (25 μM) for 24 h. In parallel, after pretreatment with crocin (5 and 10 μM) and donepezil (5 and 10 μM) for 24 h, cells were treated with H2O2 (800 μM) for 4 h. Finally, the cell viability and intracellular reactive oxygen species (ROS) generation were evaluated using AlamarBlue® and 2', 7'-dichlorodihydrofluorescein diacetate (DCFH-DA), respectively. The western blot test was done to compare the protein level of phospho SAPK/JNK, SAPK/JNK, PI3 Kinase P85, Phospho-PI3 Kinase P85, caspase-3 and cytochrome c) cyt c).
Results: Crocin and donepezil could significantly decrease the Aβ toxicity and ROS level. While treatment with Aβ increased Cyt c release from mitochondria to cytosol, cleaved form of caspase-3 (17 kDa) and activated form of SAPK/JNK p44/4 decreased the activated form of PI3 Kinase P85 protein, indicating that crocin could significantly block the apoptosis initiated with Aβ.
Conclusion: According to the results, crocin could be a promising candidate for further evaluations against the development of Alzheimer's disease through mitogen-activated protein kinases (MAPK) and the phosphatidylinositol 3-kinase (PI3K)/protein kinase B (AKT) signaling (PI3 K/AKT) pathways. -
کلید واژه
Alzheimer's disease, beta-amyloid, crocin, hydrogen peroxide, donepezil, oxidative stress.
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