The protein collagen is a major component of connective tissue and has many medical and non-medical applications. The increase in world population and the need for protein has led to a specific focus on seafood. On the other hand, the jellyfish is a rich source of collagen. Therefore, collagen extraction from jellyfish has attracted the attention of many researchers. This study aimed to extract and purify collagen from the jellyfish Catostylus mosaicus. The protein content and collagen type were evaluated using a spectrophotometer and electrophoresis, respectively. Also, the denaturation temperature of extracted collagen was measured using a viscometer. The results showed pepsin-soluble collagen extracted from the jellyfish's umbrella consists of α chains and β dimer. The yield of pepsin-soluble collagen extraction from jellyfish umbrella based on dry weight is 14.58%. Also, the results showed that the denaturation temperature of collagen extracted is about 28 . The results suggest that this jellyfish species can potentially be used as a renewable marine source of collagen instead of other sources of collagen.